Synonyms
DKFZp564M1863 antibody, PhLP antibody, PDCL antibody, phosducin-like protein antibody, phosducin-like antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
34 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 244 of PHLP (Uniprot ID# Q13371)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

PHLP antibody detects PHLP protein at cytoplasm on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
PHLP antibody (107-10666) dilution: 1:500.

PHLP antibody detects PHLP protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PHLP antibody (107-10666) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PHLP(107-10666) antibody at 1:200 dilution.

Sample(30 ug whole cell lysate)
A:H1299
10% SDS PAGE
107-10666 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.