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Anti-Phos-ABL1-Y245 Antibody
Catalog #: 131-11084
Mouse Anti-Human ABL1 (NKPTVY[pTyr245]GVSP) Monoclonal Antibody (Clone: 4H8-C3-D8)
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg, 1 mg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P00519-2 |
| Gene Id | 25 |
| Gene Symbols | ABL1, ABL, JTK7 |
| Protein Name / Synonyms | Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150 |
| Clonality | Monoclonal |
| Clone | 4H8-C3-D8 |
| Preparation | The immunogen was polypeptidet of human ABL1 (NKPTVY[pTyr245]GVSP). This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgM fraction of tissue culture supernatant was purified using a thiophilic medium with 2-mercaptopyridine. |
| Immunogen | Synthetic peptide corresponding to residues 240-249 (NKPTVYGVSP) of human ABL1, containing phosphotyrosine at position Tyr245. |
| Immunogen Sequence | NKPTVY[pTyr245]GVSP |
| Isotype | IgM |
| Recommended Applications | ELISA (recommended work dilution = 1:5, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Angiogenesis, Cardiovascular Disease, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
ABL1 is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t (9; 22) translocation results in the head-to-tail fusion of the BCR and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.
Storage/Stability
Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.