Anti-Phospho-ABL1 (Y245) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P00519
Gene Id	25
Gene Symbols	ABL1
Protein Name / Synonyms	Phospho-ABL1(Y245)
Clonality	Polyclonal
Preparation	This ABL1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y245 of human ABL1.
Concentration (lot specific)	0.7 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Angiogenesis, Cardiovascular Disease, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ABL1 is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.

Specificity

Other Names

ABL1; ABL; JTK7; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150

NCBI Accession #

NP_005148.2;NP_009297.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

122873

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50

	Western blot analysis of anti-ABL Pab (Cat# 102-15260) in mouse colon tissue lysate (35ug/lane). ABL(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Confocal immunofluorescent analysis of Phospho-ABL1-Y245 Antibody(Cat# 102-15260) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8^°C (short-term); -20^°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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