Anti-Phospho-AKT1 (S473) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P31749
Gene Id	207
Gene Symbols	AKT1
Protein Name / Synonyms	Phospho-AKT1(S473)
Clonality	Polyclonal
Preparation	This Phospho-AKT1-pS473 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S473 of human AKT1.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, Dot Blotting
Research Area	Angiogenesis, Apoptosis, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Specificity

Other Names

AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; Protein kinase B alpha; Proto-oncogene c-Akt; RAC-PK-alpha

NCBI Accession #

NP_001014431.1;NP_001014432.1;NP_005154.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

55686

Recommended Dilutions

DB: 1:500; WB: 1:1000

	Dot blot analysis of anti-AKT1-pS473 Phospho-specific Pab (Cat.102-15425) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Western blot analysis of extracts from NIH-3T3 cells,untreated or treated with PDGF,using Phospho-Akt(Ser473)(left) or Akt antibody(right).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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