Anti-Phospho-Caspase 6 (S257) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P55212
Gene Id	839
Gene Symbols	CASP6
Protein Name / Synonyms	Phospho-Caspase 6(S257)
Clonality	Polyclonal
Preparation	This Caspase 6 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S257 of human Caspase 6.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Apoptosis, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Caspase 6 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein could be processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

CASP6; MCH2; Caspase-6; Apoptotic protease Mch-2; Caspase-6 subunit p18; Caspase-6 subunit p11

NCBI Accession #

NP_001217.2;NP_116787.1

Other Accession #

O35397; O08738; Q3T0P5

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

33310

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-Phospho-Caspase-pS257 (Cat# 102-15275) in liver cell line lysates (35ug/lane). Casp6-pS257(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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