Anti-Phospho-Caspase 9 (S196) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P55211
Gene Id	842
Gene Symbols	CASP9
Protein Name / Synonyms	Phospho-Caspase 9(S196)
Clonality	Polyclonal
Preparation	This Phospho-Caspase 9-S196 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S196 of human caspase 9.
Concentration (lot specific)	0.490 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Apoptosis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.

Specificity

Other Names

CASP9; MCH6; Caspase-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6; Caspase-9 subunit p35; Caspase-9 subunit p10

NCBI Accession #

NP_001220.2;NP_001264983.1;NP_127463.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

46281

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-Phospho-Caspase 9-S196 Pab (Cat# 102-15276) is used in Western blot to detect Phospho-Caspase 9-S196 in Y79 cell line lysates.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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