Anti-Phospho-CCNB1 (S35) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P14635
Gene Id	891
Gene Symbols	CCNB1
Protein Name / Synonyms	Phospho-CCNB1(S35)
Clonality	Polyclonal
Preparation	This CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1.
Concentration (lot specific)	0.290 mg/ml
Recommended Applications	Dot Blotting, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq].

Specificity

Other Names

CCNB1; CCNB; G2/mitotic-specific cyclin-B1

NCBI Accession #

NP_114172.1

Other Accession #

NP_114172.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

48337

Recommended Dilutions

WB: 1:1100; DB: 1:500

	Western blot analysis of lysate from Hela cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody (Cat# 102-15729) or CCNB1-S9 Antibody.Lysate at 15ug per lane.102-15729 was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
	Dot blot analysis of CCNB1 Antibody (Phospho S35) Phospho-specific Pab (Cat# 102-15729) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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