Anti-Phospho-CDC25A (S293) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P30304
Gene Id	993
Gene Symbols	CDC25A
Protein Name / Synonyms	Phospho-CDC25A(S293)
Clonality	Polyclonal
Preparation	This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S293 of human CDC25A.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Specificity

Other Names

CDC25A; M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A

NCBI Accession #

NP_001780.2;NP_963861.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

59087

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-Phospho-CDC25A-S293 Pab (Cat# 102-15280) is used in Western blot to detect Phospho-CDC25A-S293 in cells transfected with wild type or mutant S293A of CDC25A. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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