Anti-Phospho-DAXX (S213) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9UER7
Gene Id	1616
Gene Symbols	DAXX
Protein Name / Synonyms	Phospho-DAXX(S213)
Clonality	Polyclonal
Preparation	This DAXX Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S213 of human DAXX.
Concentration (lot specific)	0.460 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

DAXX is proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. DAXX seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. This protein down-regulates basal and activated transcription, and it also seems to act as a transcriptional co-repressor and inhibits PAX3 and ETS1 through direct protein-protein interaction. DAXX modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

DAXX; BING2; DAP6; Death domain-associated protein 6; Daxx; ETS1-associated protein 1; Fas death domain-associated protein

NCBI Accession #

NP_001135441.1;NP_001241646.1;NP_001341.1

Other Accession #

Q8VIB2; O35613

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

81373

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-Phospho-DAXX-S213 Pab (Cat#AP73082a) is used in Western blot to detect Phospho-DAXX-S213 in mouse thymus tissue lysate
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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