Anti-Phospho-EGFR (Y1172) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P00533
Gene Id	1956
Gene Symbols	EGFR
Protein Name / Synonyms	Phospho-EGFR(Y1172)
Clonality	Polyclonal
Preparation	This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1172 of human EGFR.
Concentration (lot specific)	0.410 mg/ml
Recommended Applications	Western Blotting, Dot Blotting
Research Area	Cardiovascular Disease, Cancer, Inflammation, HER/ErbB Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. EGFR is a receptor for EGF, but also for other members of the EGF family, including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.

Specificity

Other Names

EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

NCBI Accession #

NP_005219.2;NP_958439.1;NP_958440.1;NP_958441.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

134277

Recommended Dilutions

WB: 1:1000; DB: 1:500

	Western blot analysis of extracts from A431 cells, untreated or treated with EGF, using phospho EGFR-Y1172 (left) or ErBB2 antibody (right)
	Western blot analysis of EGFR (arrow) in Hela cell lysates, either induced (Lane 1) or noninduced with EGF (Lane 2).
	Dot blot analysis of anti-EGFR-pY1172 Phospho-specific Pab (Cat# 102-15412) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Western blot analysis of extracts from A431 cells,untreated or treated with EGF using Phospho-EGFR(Tyr1172)(left) or EGFR antibody(right).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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