Anti-Phospho-ERBB2 (Y1196) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04626
Gene Id	2064
Gene Symbols	ERBB2
Protein Name / Synonyms	Phospho-ERBB2(Y1196)
Clonality	Polyclonal
Preparation	This ERBB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1196 of human ERBB2.
Concentration (lot specific)	0.350 mg/ml
Recommended Applications	IF, Dot Blotting, Western Blotting
Research Area	Angiogenesis, Cardiovascular Disease, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Specificity

Other Names

ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen=CD340; Flags: Precursor

NCBI Accession #

NP_001005862.1;NP_004439.2

Other Accession #

NP_001005862.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

137910

Recommended Dilutions

WB: 1:4000; IF: 1:100; DB: 1:500

	Western blot analysis of extracts from A431 cells,untreated or treated with EGF,100ng/ml?using phospho ERBB2 Y1196 (left) or ERBB2 antibody(right)
	Fluorescent confocal image of MCF7 cells stained with phospho-ERBB2-Y1196 antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15601 phospho-ERBB2- Y1196 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the phospho-ERBB2-Y1196 to the plasma membrane and cytoplasm.
	Dot blot analysis of ERBB2 Antibody (Phospho Y1196) Phospho-specific Pab (Cat# 102-15601) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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