Anti-Phospho-ErbB2 (Y1248) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04626
Gene Id	2064
Gene Symbols	ERBB2
Protein Name / Synonyms	Phospho-ErbB2(Y1248)
Clonality	Polyclonal
Preparation	This ErbB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1248 of human ErbB2.
Concentration (lot specific)	0.320 mg/ml
Recommended Applications	Dot Blotting, Western Blotting
Research Area	Angiogenesis, Cardiovascular Disease, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ErbB2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors.

Specificity

Other Names

Receptor tyrosine-protein kinase erbB-2 [Precursor]; p185erbB2; C-erbB-2; NEU proto-oncogene; Tyrosine kinase-type cell surface receptor HER2; MLN 19; ERBB2; HER2; NEU; NGL;

NCBI Accession #

NP_001005862.1;NP_004439.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

137910

Recommended Dilutions

WB: 1:8000; DB: 1:500

	Western blot analysis of extracts from A431 cells,untreated or treated with EGF,100ng/ml , using phospho-ERBB2-pY1248(L)(left) or ERBB2 antibody(right)
	Western blot analysis of extracts from A431 cells,untreated or treated with EGF,100ng/ml , using phospho-ERBB2-Y1248(left) or ERBB2 antibody(right)
	Dot blot analysis of anti-Phospho-ErbB2-pY1248(M) Phospho-specific Pab (Cat# 102-15298) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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