Anti-Phospho-ERBB3 (Y1289) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P21860
Gene Id	2065
Gene Symbols	ERBB3
Protein Name / Synonyms	Phospho-ERBB3(Y1289)
Clonality	Polyclonal
Preparation	This ERBB3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1289 of human ERBB3.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, Dot Blotting
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form.

Specificity

Other Names

ERBB3; HER3; Receptor tyrosine-protein kinase erbB-3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3

NCBI Accession #

NP_001005915.1;NP_001973.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

148098

Recommended Dilutions

DB: 1:500; WB: 1:1000

	Dot blot analysis of anti-Phospho-ERBB3 (Tyr1289) Antibody Phospho-specific Pab (Cat# 102-15526) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Western blot analysis of extracts from T47D cells,untreated or treated with NRG using Phospho-ErBB3(Tyr1289)(left) or ErBB3 antibody(right).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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