Anti-Phospho-GATA6 (Y271) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q92908
Gene Id	2627
Gene Symbols	GATA6
Protein Name / Synonyms	Phospho-GATA6(Y271)
Clonality	Polyclonal
Preparation	This Phospho-GATA6-Y271 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide surrounding Y271 of human GATA6.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IF, Dot Blotting
Research Area	Angiogenesis, Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Thought to be important for regulating terminal differentiation and/or proliferation.

Specificity

Other Names

GATA6; Transcription factor GATA-6; GATA-binding factor 6

NCBI Accession #

NP_005248.2

Other Accession #

NP_005248.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

60033

Recommended Dilutions

IF: 1:100; DB: 1:500

Fluorescent confocal image of HepG2 cells stained with phospho-GATA6-Y271 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15558 phospho-GATA6-Y271 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the phospho-GATA6 immunosignal mainly to the nucleus.

Dot blot analysis of Phospho-GATA6-pY271 Phospho-specific Pab (Cat# 102-15558) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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