Anti-Phospho-HER4 (Y1162) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q15303
Gene Id	2066
Gene Symbols	ERBB4
Protein Name / Synonyms	Phospho-HER4(Y1162)
Clonality	Polyclonal
Preparation	This HER4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1162 of human HER4.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IHC-P, Western Blotting, IF
Research Area	Cardiovascular Disease, Cancer, HER/ErbB Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

HER4/ERBB4 is a member of the type I receptor tyrosine kinase subfamily that includes EGFR, ERBB2 and ERBB3. It is a receptor for NDF/heregulin.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

ERBB4; HER4; Receptor tyrosine-protein kinase erbB-4; Proto-oncogene-like protein c-ErbB-4; Tyrosine kinase-type cell surface receptor HER4; p180erbB4; ERBB4 intracellular domain; s80HER4

NCBI Accession #

NP_001036064.1;NP_005226.1

Other Accession #

Q62956; Q61527

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

146808

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50

	Phospho-HER4-Y1162 Antibody (cat# 102-15306)was used to detect Phospho-HER4-Y1162 in FG pancreatic carcinoma cell line lysate. FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min Phospho-HER4-Y1162 Antibody (cat# 102-15306) was used at 1:750 in 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Confocal immunofluorescent analysis of Phospho-HER4-Y1162 Antibody(Cat# 102-15306) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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