Anti-Phospho-HIST1H3B3 (S10) Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P68431
Gene Id	8968
Gene Symbols	HIST1H3A
Protein Name / Synonyms	Phospho-HIST1H3B3(S10)
Clonality	Polyclonal
Preparation	This HIST1H3B3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human HIST1H3B3.
Concentration (lot specific)	0.430 mg/ml
Recommended Applications	Western Blotting, IHC-P, Dot Blotting
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Specificity

Other Names

HIST1H3A; H3FA; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l

NCBI Accession #

NP_003520.1;NP_003521.2;NP_003522.1;NP_003523.1;NP_003524.1;NP_003525.1;NP_003526.1;NP_003527.1;NP_003528.1;NP_066298.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

15404

Recommended Dilutions

WB: 1:2000; DB: 1:500; IHC: 1:50-100

	Western blot analysis of extracts from Hela cells,untreated or treated with FBS and Calyculin A ,100nM, using phospho-HIST1H3B3-S10(left) or HIST1H3B3 antibody(right)
	Western blot analysis of extracts from Hela cells,untreated or treated with FBS and Calyculin A,100nM, using phospho-H3-Ps10(left) or H3 antibody(right)
	Dot blot analysis of anti-Phospho-HIST1H3B3-S10 Phospho-specific Pab (Cat# 102-15304) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products