Anti-Phospho-IKKb (Y199) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O14920
Gene Id	3551
Gene Symbols	IKBKB
Protein Name / Synonyms	Phospho-IKKb(Y199)
Clonality	Polyclonal
Preparation	This IKKb Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y199 of human IKKb.
Concentration (lot specific)	0.460 mg/ml
Recommended Applications	Western Blotting
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the inhibitory I-kappa-B proteins by kinases (IKKa or IKKb) leads to the dissociation of the inhibitor/NF-kappa-B complex and marks the inhibitors for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B kinase 2; Nuclear factor NF-kappa-B inhibitor kinase beta

NCBI Accession #

NP_001177649.1;NP_001229707.1;NP_001547.1

Other Accession #

Q9QY78; O88351; Q95KV0

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

86564

Recommended Dilutions

WB: 1:1000

The anti-Phospho-IKKb-Y199 Pab (Cat# 102-15308) is used in Western blot to detect Phospho-IKKb-Y199 in Y79 tissue lysate

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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