Rabbit Anti-Human Phospho-KDR/FLK1(Y996) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P35968 |
| Gene Id | 3791 |
| Gene Symbols | KDR |
| Protein Name / Synonyms | Phospho-KDR/FLK1(Y996) |
| Clonality | Polyclonal |
| Preparation | This KDR/FLK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y996 of human KDR/FLK1. |
| Concentration (lot specific) | 0.630 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Angiogenesis, Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
KDR (FLK1) is a receptor for VEGF or VEGFC. This protein has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.
Specificity
Other Names
KDR; FLK1; VEGFR2; Vascular endothelial growth factor receptor 2; Fetal liver kinase 1; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; CD_antigen=CD309; Flags: Precursor
NCBI Accession #
NP_002244.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
151527
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Anti-Phospho-KDR/FLK1-Y996 Pab (Cat# 102-15302) is used in Western blot to detect Phospho-KDR/FLK1-Y996 in HMVEC cell line lysate. Endothelial cells were stimulated with 50ug/ml VEGF for 5min; 20ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with Phospho-KDR/FLK1-Y996 Antibody (102-15302)diluted to 1:500 in 1% Milk/TBST. Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.