Anti-Phospho-LIN28 (S134) Antibody

Catalog #: 102-15537

Rabbit Anti-Human Phospho-LIN28(S134) Antibody, 400 ul

Specifications

Description

Introduction

LIN28 is probable transcription factor. It plays a critical role in the control of immune response.

Specificity

Other Names

LIN28A; CSDD1; LIN28; ZCCHC1; Protein lin-28 homolog A; Zinc finger CCHC domain-containing protein 1

NCBI Accession #

NP_078950.1

Other Accession #

NP_078950

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

22743

Recommended Dilutions

IF: 1:100; DB: 1:500

Fluorescent confocal image of SY5Y cells stained with phospho- LIN28- S134 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15537 phospho- LIN28- S134 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the phospho- LIN28 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000131914).

Dot blot analysis of anti-Phospho-LIN28-S134 Phospho-specific Pab (Cat# 102-15537) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.