Anti-Phospho-MUC1 (T1224) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P15941
Gene Id	4582
Gene Symbols	MUC1
Protein Name / Synonyms	Phospho-MUC1(T1224)
Clonality	Polyclonal
Preparation	This MUC1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T1224 of human MUC1.
Concentration (lot specific)	0.400 mg/ml
Recommended Applications	Dot Blotting, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MUC1 is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas.

Specificity

Other Names

MUC1; PUM; Mucin-1; Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; PEMT; Peanut-reactive urinary mucin; Polymorphic epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin; CD_antigen=CD227; Mucin-1 subunit alpha; Mucin-1 subunit beta; MUC1-CT

NCBI Accession #

NP_001018016.1;NP_001018017.1;NP_001037855.1;NP_001037856.1;NP_001037857.1;NP_001037858.1;NP_001191214.1;NP_001191215.1;NP_001191216.1;NP_001191217.1;NP_001191218.1;NP_001191219.1;NP_001191220.1;NP_00

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

122102

Recommended Dilutions

DB: 1:500; IF: 1:10-50

	Dot blot analysis of anti-Phospho-MUC1-pT1224 Phospho-specific Pab (Cat# 102-15535) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Confocal immunofluorescent analysis of Phospho-MUC1-T1224 Antibody(Cat# 102-15535) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products