Anti-Phospho-MYT1 (T495) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q99640
Gene Id	9088
Gene Symbols	PKMYT1
Protein Name / Synonyms	Phospho-MYT1(T495)
Clonality	Polyclonal
Preparation	This MYT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T495 of human MYT1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, Dot Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Specificity

Other Names

PKMYT1; MYT1; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase

NCBI Accession #

NP_001245379.1;NP_001245380.1;NP_004194.3;NP_872629.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

54521

Recommended Dilutions

DB: 1:500; WB: 1:1000; IHC: 1:50-100

	Dot blot analysis of anti-Phospho-MYT1-T495 Antibody (Cat# 102-15319) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	The anti-Phospho-MYT1-T495 Antibody (Cat# 102-15319) is used in Western blot to detect Phospho-MYT1-T495 in HL60 (left) and SK-BR-3 (right) tissue lysates.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products