Rabbit Anti-Human Phospho-p16-INK4A(S140) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P42771 |
| Gene Id | 1029 |
| Gene Symbols | CDKN2A |
| Protein Name / Synonyms | Phospho-p16-INK4A(S140) |
| Clonality | Polyclonal |
| Preparation | This p16-INK4A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S140 of human p16-INK4A. |
| Concentration (lot specific) | 0.310 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
p16-INK4A functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. This protein acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. The gene for this protein is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
Specificity
Other Names
CDKN2A; CDKN2; MTS1; Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; Multiple tumor suppressor 1; p16-INK4a
NCBI Accession #
NP_000068.1;NP_001182061.1;NP_478104.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
16533
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | The anti-Phospho-p16-INK4A-S140 Pab (Cat# 102-15322) is used in Western blot to detect Phospho-p16-INK4A-S140 in A2058 tissue lysate |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.