Anti-Phospho-P53 (S9) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04637
Gene Id	7157
Gene Symbols	TP53
Protein Name / Synonyms	Phospho-P53(S9)
Clonality	Polyclonal
Preparation	This P53 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S9 of human P53.
Concentration (lot specific)	0.250 mg/ml
Recommended Applications	Dot Blotting, Western Blotting, IF
Research Area	Apoptosis, Cancer, p53 Signaling, Autophagy, DNA Damage
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.

Specificity

Other Names

TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

NCBI Accession #

NP_000537.3;NP_001119584.1;NP_001119585.1;NP_001119586.1;NP_001119587.1;NP_001119588.1;NP_001119589.1;NP_001119590.1;NP_001263624.1;NP_001263625.1;NP_001263626.1;NP_001263627.1;NP_001263628.1;NP_00126

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

43653

Recommended Dilutions

DB: 1:500; WB: 1:1000; IF: 1:10-50

	Dot blot analysis of anti-Phospho-P53-S9 Antibody (Cat# 102-15337) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.
	Western blot analysis of Phospho-P53-S9 (Cat# 102-15337) in HL60(left) and Ramos (right) cell line lysate.
	Confocal immunofluorescent analysis of Phospho-P53-S9 Antibody(Cat# 102-15337) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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