Anti-Phospho-PDPK1 (S396) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O15530
Gene Id	5170
Gene Symbols	PDPK1
Protein Name / Synonyms	Phospho-PDPK1(S396)
Clonality	Polyclonal
Preparation	This PDPK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S396 of human PDPK1.
Concentration (lot specific)	0.400 mg/ml
Recommended Applications	Dot Blotting
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

Specificity

Other Names

PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1

NCBI Accession #

NP_001248745.1;NP_002604.1;NP_112558.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

63152

Recommended Dilutions

DB: 1:500

Dot blot analysis of anti-PDPK1-pS396 Phospho-specific Pab (RB13093) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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