Anti-Phospho-PDX1 (T11) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P52945
Gene Id	3651
Gene Symbols	PDX1
Protein Name / Synonyms	Phospho-PDX1(T11)
Clonality	Polyclonal
Preparation	This PDX1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T11 of human PDX1.
Concentration (lot specific)	0.25 mg/ml
Recommended Applications	IF, Dot Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PDX1 is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this protein are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4).

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

PDX1; IPF1; Pancreas/duodenum homeobox protein 1; Glucose-sensitive factor; Insulin promoter factor 1; Insulin upstream factor 1; Islet/duodenum homeobox-1; Somatostatin-transactivating factor 1

NCBI Accession #

NP_000200.1

Other Accession #

P52947; P52946; NP_000200

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

30771

Recommended Dilutions

IF: 1:100; DB: 1:500

Fluorescent confocal image of SY5Y cells stained with phospho-PDX1-T11 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15512 phospho-PDX1-T11 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the phospho-PDX1 immunosignal mainly to the nucleus.

Dot blot analysis of PDX1 Antibody (T11) Pab (Cat# 102-15512) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8^°C (short-term); -20^°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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