Anti-Phospho-RAD9 (S272) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q99638
Gene Id	5883
Gene Symbols	RAD9A
Protein Name / Synonyms	Phospho-RAD9(S272)
Clonality	Polyclonal
Preparation	This RAD9 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S272 of human RAD9.
Concentration (lot specific)	0.270 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Rad9 is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade.

Specificity

Predicted Reactivity

Monkey

Other Names

RAD9A; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A

NCBI Accession #

NP_001230153.1;NP_004575.1

Other Accession #

Q4R5X9

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

42547

Recommended Dilutions

WB: 1:1000; IHC: 1:10-100

	The anti-Phospho-Rad9-S272 Pab (Cat# 102-15339) is used in Western blot to detect Phospho-Rad9-S272 in HL60 (left), Hela(middle), and Y79(right)tissue lysates.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products