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Anti-Phospho-SEPARIN (S1126) Antibody
Catalog #: 102-15351
Rabbit Anti-Human Phospho-SEPARIN(S1126) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q14674 |
| Gene Id | 9700 |
| Gene Symbols | ESPL1 |
| Protein Name / Synonyms | Phospho-SEPARIN(S1126) |
| Clonality | Polyclonal |
| Preparation | This SEPARIN Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1126 of human SEPARIN. |
| Concentration (lot specific) | 0.373 mg/ml |
| Recommended Applications | IHC-P, Dot Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
The metaphase-to-anaphase transition is the final discrete event in duplication and separation of the genetic material of the cell. Its timing is regulated by the activation of the anaphase-promoting complex (APC). In both budding and fission yeast, the degradation of the Pds1 or Cut2 protein, respectively, is required for the onset of sister chromatid separation. Both proteins are APC substrates. Pds1 and Cut2 proteins associate with the yeast separin proteins Esp1 and Cut1, respectively, and prevent the separins from promoting chromatid separation. Pds1 and Cut2 are also called anaphase inhibitors or securins
Specificity
Other Names
ESPL1; ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase
NCBI Accession #
NP_036423.4
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
233175
Recommended Dilutions
DB: 1:500; IHC: 1:50-100
 | Dot blot analysis of anti-hSeparase-S801 Phospho-specific Pab (Cat# 102-15351) on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.