Anti-Phospho-SMAD2 (S118) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q15796
Gene Id	4087
Gene Symbols	SMAD2
Protein Name / Synonyms	Phospho-SMAD2(S118)
Clonality	Polyclonal
Preparation	This SMAD2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S118 of human SMAD2.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IF, Dot Blotting
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein belongs to the SMAD, a family of proteins similar to the proteins of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Drosophila, Pig

Other Names

SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; JV18-1; Mad-related protein 2; SMAD family member 2

NCBI Accession #

NP_001003652.1;NP_005892.1

Other Accession #

O54835; Q9JIW5; O15198; Q9R1V3; P97454; Q99717; Q9W7E7; Q56I99; P84025; P84024; Q8BUN5; P84022; P84023; O70436; Q62432; Q9I9P9; Q1W668; P97588; P70340; Q15797; Q9I8V2; Q1JQA2; P42003

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

52306

Recommended Dilutions

IF: 1:10-50; DB: 1:500

	Confocal immunofluorescent analysis of Phospho-SMAD2-S118 Antibody(Cat# 102-15507) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
	Dot blot analysis of anti-Phospho-SMAD2-S118 Antibody (Cat# 102-15507) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products