Anti-Phospho-SMAD2 (T220) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q15796
Gene Id	4087
Gene Symbols	SMAD2
Protein Name / Synonyms	Phospho-SMAD2(T220)
Clonality	Polyclonal
Preparation	This SMAD2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T220 of human SMAD2.
Concentration (lot specific)	0.28 mg/ml
Recommended Applications	Western Blotting, IF, Dot Blotting
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SMAD2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF?receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smad's; and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish, Bovine

Other Names

SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; JV18-1; Mad-related protein 2; SMAD family member 2

NCBI Accession #

NP_001003652.1;NP_005892.1

Other Accession #

O70436; Q62432; Q9I9P9; Q1W668

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Buffer

PBS with 0.09% sodium azide

Calculated Molecular Weight (Da)

52306

Recommended Dilutions

WB: 1:500; IF: 1:10-50; DB: 1:500

	Western blot analysis of extracts from Hela cell,untreated or treated with PMA,using phospho-SMAD2-T220(left) or SMAD2 antibody(right).
	Confocal immunofluorescent analysis of Phospho-SMAD2-T220 Antibody(Cat# 102-15515) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
	Dot blot analysis of anti-Phospho-SMAD2-T220 Phospho-specific Pab (Cat# 102-15515??) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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