Anti-Phospho-SMAD3 (S208) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P84022
Gene Id	4088
Gene Symbols	SMAD3
Protein Name / Synonyms	Phospho-SMAD3(S208)
Clonality	Polyclonal
Preparation	This SMAD3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S208 of human SMAD3.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	IF, Dot Blotting
Research Area	Cardiovascular Disease, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.

Specificity

Predicted Reactivity

Mouse, Rat, Chicken, Pig

Other Names

SMAD3; MADH3; Mothers against decapentaplegic homolog 3; JV15-2; SMAD family member 3

NCBI Accession #

NP_001138574.1;NP_001138575.1;NP_001138576.1;NP_005893.1

Other Accession #

P84025; P84024; Q8BUN5; P84023

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

48081

Recommended Dilutions

IF: 1:10-50; DB: 1:500

	Confocal immunofluorescent analysis of Phospho-SMAD3-S208 Antibody(Cat# 102-15352) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
	Dot blot analysis of anti-hSMAD3-S208 Phospho-specific Pab (Cat# 102-15352) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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