Anti-Phospho-SMAD3 (S213) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P84022
Gene Id	4088
Gene Symbols	SMAD3
Protein Name / Synonyms	Phospho-SMAD3(S213)
Clonality	Polyclonal
Preparation	This SMAD3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S213 of human SMAD3.
Concentration (lot specific)	0.450 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Cardiovascular Disease, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.

Specificity

Predicted Reactivity

Mouse, Rat, Chicken, Pig

Other Names

SMAD3; MADH3; Mothers against decapentaplegic homolog 3; JV15-2; SMAD family member 3

NCBI Accession #

NP_001138574.1;NP_001138575.1;NP_001138576.1;NP_005893.1

Other Accession #

P84025; P84024; Q8BUN5; P84023

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

48081

Recommended Dilutions

IHC: 1:50-100; WB: 1:1000

	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	The anti-Phospho-SMAD3-S213 Pab (Cat# 102-15353) is used in Western blot to detect Phospho-SMAD3-S213 in Ramos tissue lysate

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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