Anti-phospho-Sox2 (S246) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P48431
Gene Id	6657
Gene Symbols	SOX2
Protein Name / Synonyms	phospho-Sox2(S246)
Clonality	Polyclonal
Preparation	This Sox2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S246 of human Sox2.
Concentration (lot specific)	0.450 mg/ml
Recommended Applications	IF, Dot Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Specificity

Predicted Reactivity

Mouse

Other Names

SOX2; Transcription factor SOX-2

NCBI Accession #

NP_003097.1

Other Accession #

P48432; NP_003097.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

34310

Recommended Dilutions

IF: 1:200; DB: 1:500

Fluorescent confocal image of SY5Y cells stained with phospho-Sox2- S246 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15551 phospho-Sox2- S246 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the phospho-Sox2 immunosignal mainly to the nucleus.

Dot blot analysis of anti-phospho-Sox2-pS246 Phospho-specific Pab (Cat# 102-15551) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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