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Anti-Phospho-STAT1 (S727) Antibody
Catalog #: 102-15357
Rabbit Anti-Human Phospho-STAT1(S727) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P42224 |
| Gene Id | 6772 |
| Gene Symbols | STAT1 |
| Protein Name / Synonyms | Phospho-STAT1(S727) |
| Clonality | Polyclonal |
| Preparation | This STAT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT1. |
| Concentration (lot specific) | 0.35 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Angiogenesis, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.
Specificity
Predicted Reactivity
Mouse, Pig
Other Names
STAT1; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84
NCBI Accession #
NP_009330.1;NP_644671.1
Other Accession #
Q764M5; P42225
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
87335
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-Phospho-STAT1 Pab (Cat# 102-15357) in mouse brain tissue lysate (35ug/lane). Phospho-STAT1(arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.