Anti-Phospho-STAT3 (S727) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Host Species	Rabbit
Accession Number	P40763
Gene Id	6774
Gene Symbols	STAT3
Protein Name / Synonyms	Phospho-STAT3(S727)
Clonality	Polyclonal
Preparation	This STAT3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT3.
Concentration (lot specific)	0.45 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Angiogenesis, Cancer, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.

Specificity

Other Names

STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor

NCBI Accession #

NP_003141.2;NP_644805.1;NP_998827.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

88068

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:50-100

Fluorescent confocal image of SY5Y cells stained with phospho-STAT3-S727 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15358 phospho-STAT3-S727 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the phospho-STAT3 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610).

Antigen Source

HUMAN

Storage/Stability

2-8^°C (short-term); -20^°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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