Anti-Phospho-STAT5a (Y694) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P42229
Gene Id	6776
Gene Symbols	STAT5A
Protein Name / Synonyms	Phospho-STAT5a(Y694)
Clonality	Polyclonal
Preparation	This STAT5a Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y694 of human STAT5a.
Concentration (lot specific)	0.400 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

STAT5a is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this protein is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells.

Specificity

Other Names

STAT5A; STAT5; Signal transducer and activator of transcription 5A

NCBI Accession #

NP_003143.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

90647

Recommended Dilutions

WB: 1:1000; IF: 1:200; IHC: 1:50-100

	Western blot analysis of anti-Phospho-STAT5 Pab(Cat# 102-15361) in HepG2 cell line lysate (35ug/lane). Phospho-STAT5(arrow) was detected using the purified Pab.
	Fluorescent confocal image of an SY5Y cell stained with 102-15361 Phospho-STAT5a antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with 102-15361 Phospho-STAT5a primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min)(not shown). Phospho-STAT5a immunoreactivity is localized exclusively to the nucleus.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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