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Anti-Phospho-Tau (pThr231) Antibody, Biotinylated
Catalog #: RB-02-0021B-50
Mouse anti-Phosphorylated-Tau (pThr231) Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Specificity | The antibody secreted by clone 7B10.F2.E5 specifically detected phosphorylated Tau protein derived from human brain tissue. It recognized PHF-tau phosphorylated at Thr231.In both ELISA and WB, this antibody shows no cross-reactivity with other unrelated proteins. |
| Host Species | Mouse |
| Accession Number | NP_058518 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was a synthetic peptide derived from human Tau. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells derived from the immunized mouse. The IgM fraction of cell culture supernatant was purified by affinity chromatography. |
| Isotype | IgM |
| Recommended Applications | ELISA (recommended work dilution= 1: 5, 000-10, 000), Western Blotting (recommended work dilution= 2-5 µg/ml), Immunoprecipitation (recommended work dilution= determined by customer) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Microtubule-associated protein, tau is abnormally hyperphosphorylated in the brain of patients with Alzheimer's disease, and is the major protein subunit of paired helical filaments. There is also a significant pool of non-paired helical filament abnormally phosphorylated tau in Alzheimer's disease brain. Tau is a family of six isoforms, derived from a single gene by mRNA splicing. Tau protein is produced by a single gene expressed predominantly in neurons. They vary in size from 352-441 amino acides. In Alzheimer disease Tau is hyperphosphorylated, containing 3-4 fold more phosphoate/mole of the protein than the normal tau. Recent investigations show that MARK and PKA phosphorylate several sites within the repeats (notably the KXGS motifs including Ser262, Ser324, and Ser356, plus Ser320); in addition PKA phosphorylates some sites in the flanking domains, notably Ser214. This type of phosphorylation strongly reduces tau's affinity for microtubules, and at the same time inhibits tau's assembly into PHFs (Paired helical filament).
Typical Data
Storage/Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.
References
- Arima, K., et al. (2000). NACP/alpha-synuclein and tau constitute two distinctive subsets of filaments in the same neuronal inclusions in brains from a family of parkinsonism and dementia with Lewy bodies: double-immunolabeling fluorescence and electron microscope studies. Acta Neuropathol. 100(2), 115-121.
- Gong CX, Grundke-Iqbal I, Iqbal K(1994).Dephosphorylation of Alzheimer's disease abnormally phosphorylated tau by protein phosphatase-2A.Neuroscience. 61(4):765-72.
- Biernat, J., et al. (1992). The switch of tau protein to an Alzheimer-like state includes the phosphorylation of two serine-proline motifs upstream of the microtubule binding region. EMBO J. 11(4), 1593-1597.