Anti-Phospho-TNFR (S274) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P19438
Gene Id	7132
Gene Symbols	TNFRSF1A
Protein Name / Synonyms	Phospho-TNFR(S274)
Clonality	Polyclonal
Preparation	This TNFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S274 of human TNFR.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Apoptosis, Cancer, Inflammation, Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

A member of the TNF-receptor superfamily, this protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease.

Specificity

Other Names

Tumor necrosis factor receptor superfamily member 1A [Precursor]; p60; TNF-R1; TNF-RI; TNFR-I; p55; CD120a antigen; TNFRSF1A; TNFAR; TNFR1;

NCBI Accession #

NP_001056.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

50495

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of hTNFR-pS274 (Cat# 102-15363) in MDA-MB468 cell line lysates (35ug/lane). TNFR (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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