Anti-Phospho-ULK1 (S556) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O75385
Gene Id	8408
Gene Symbols	ULK1
Protein Name / Synonyms	Phospho-ULK1(S556)
Clonality	Polyclonal
Preparation	This ULK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S556 of human ULK1.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	IF, Dot Blotting
Research Area	Cancer, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Involved in autophagy. Required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. Involved in axon growth (By similarity). Plays an essential role in neurite extension of cerebellar granule cells (By similarity).

Specificity

Other Names

ULK1; KIAA0722; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; Unc-51-like kinase 1

NCBI Accession #

NP_003556.1

Other Accession #

NP_003556.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

112631

Recommended Dilutions

IF: 1:200; DB: 1:500

Fluorescent image of U251 cells stained with ULK1 (phospho S556) antibody. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15662 ULK1 (phospho S556) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). ULK1 (phospho S556) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Dot blot analysis of ULK1 Antibody (Phospho S556) Phospho-specific Pab (Cat# 102-15662) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products