Anti-Phospho-YAP (S127) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P46937
Gene Id	10413
Gene Symbols	YAP1
Protein Name / Synonyms	Phospho-YAP(S127)
Clonality	Polyclonal
Preparation	This YAP Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S127 of human YAP.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Dot Blotting
Research Area	Cardiovascular Disease, Hippo Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).

Specificity

Predicted Reactivity

Mouse, Rat, Chicken

Other Names

YAP1; YAP65; Yorkie homolog; 65 kDa Yes-associated protein

NCBI Accession #

NP_001123617.1;NP_001181973.1;NP_001181974.1;NP_001269027.1;NP_001269028.1;NP_001269029.1;NP_001269030.1

Other Accession #

Q2EJA0; P46938; P46936; NP_001123617.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

54462

Recommended Dilutions

DB: 1:500

Dot blot analysis of Phospho-YAP-S127 antibody Phospho-specific Pab (Cat# 102-15559) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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