Anti-PI3KC3 (N-term G24) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q8NEB9
Gene Id	5289
Gene Symbols	PIK3C3
Protein Name / Synonyms	PI3KC3 (N-term G24)
Clonality	Polyclonal
Preparation	This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-41 amino acids from the N-terminal region of human PI3KC3.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

Specificity

Predicted Reactivity

Mouse, Rat, Pig, Xenopus

Other Names

PIK3C3; VPS34; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34

NCBI Accession #

NP_002638.2

Other Accession #

Q6AZN6; O88763; Q5D891; Q6PF93

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

101549

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	Western blot analysis of PI3KC3 Antibody (N-term G24)(Cat# 102-17757) in HeLa cell lysate. PI3KC3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with hPI3KC3 (N-term) (Cat# 102-13885), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of PI3KC3 Antibody (N-term G24)(Cat# 102-13885) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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