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Anti-PI3KCA (Center) Antibody
Catalog #: 102-17759
Rabbit Anti-Human PI3KCA (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P42336 |
| Gene Id | 5290 |
| Gene Symbols | PIK3CA |
| Protein Name / Synonyms | PI3KCA (Center) |
| Clonality | Polyclonal |
| Preparation | This PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 504-533 amino acids from the Central region of human PI3KCA. |
| Concentration (lot specific) | 1.570 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Angiogenesis, Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Specificity
Other Names
PIK3CA; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA
NCBI Accession #
NP_006209.2
Other Accession #
Q14CW1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
124284
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-PIK3CA Antibody (Center) Pab (Cat# 102-17759) in K562 cell line lysates (35ug/lane). PIK3CA (arrow) was detected using the purified Pab |
 | Western blot analysis of anti-PI3KCA Antibody (Center) Pab (Cat# 102-17759) in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane). PI3KCA (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody (Center)(Cat# 102-17759), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.