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Anti-PIAS3 (N-term) Antibody
Catalog #: 102-11578
Rabbit Anti-Human PIAS3 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9Y6X2 |
| Gene Id | 10401 |
| Gene Symbols | PIAS3 |
| Protein Name / Synonyms | PIAS3 (N-term) |
| Clonality | Polyclonal |
| Preparation | This PIAS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-126 amino acids from the N-terminal region of human PIAS3. |
| Concentration (lot specific) | 1.290 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
PIAS3 is a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor, catalyzing the covalent attachment of a SUMO protein to specific target substrates. PIAS3 plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
PIAS3; E3 SUMO-protein ligase PIAS3; Protein inhibitor of activated STAT protein 3
NCBI Accession #
NP_006090.2
Other Accession #
O70260; O54714
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
68017
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-PIAS3 Pab (Cat# 102-11578) in HL60 cell line lysate (35ug/lane). PIAS3(arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.