Synonyms
FLJ22667 antibody, MGC125361 antibody, MGC125362 antibody, PIGR antibody, polymeric immunoglobulin receptor antibody, hepatocellular carcinoma associated protein TB6 antibody, hepatocellular carcinoma-associated protein TB6 antibody, poly-Ig receptor antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
83 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 180 and 459 of PIGR (Uniprot ID# P01833)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HepG2, using PIGR(107-10904) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using PIGR(107-10904) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10904 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.