Anti-PIK3R2 (Y467) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O00459
Gene Id	5296
Gene Symbols	PIK3R2
Protein Name / Synonyms	PIK3R2 (Y467)
Clonality	Polyclonal
Preparation	This PIK3R2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from human PIK3R2.
Concentration (lot specific)	0.310 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. PIK3R2 binds to activated Protein Tyrosine Kinases, which are phosphorylated, through its SH2 domain, and acts as an adaptor, mediating the association of the P110 catalytic unit to the plasma membrane.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

PIK3R2; Phosphatidylinositol 3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta

NCBI Accession #

NP_005018.1

Other Accession #

Q63788; O08908; P23726

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

81545

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of PIK3R2 (arrow) using rabbit polyclonal PIK3R2 Antibody (Y467) (RB13091). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R2 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human brain tissue reacted with PIK3R2-pY467, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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