3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-PIM1 (C-term) Antibody
Catalog #: 102-17707
Rabbit Anti-Human PIM1 (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P11309 |
| Gene Id | 5292 |
| Gene Symbols | PIM1 |
| Protein Name / Synonyms | PIM1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This PIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-404 amino acids from the C-terminal region of human PIM1. |
| Concentration (lot specific) | 1.370 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
PIM1, which belongs to the Serine/Threonine protein kinase family, is thought to play a role in signal transduction in blood cells. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer. It may affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. PIM1 is expressed primarily in cells of the hematopoietic and germ line lineages.
Specificity
Predicted Reactivity
Bovine
Other Names
PIM1; Serine/threonine-protein kinase pim-1
NCBI Accession #
NP_001230115.1;NP_002639.1
Other Accession #
Q9N0P9
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
45412
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-PIM1 Pab (Cat# 102-17707) in A549 cell lysate. PIM1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
 | Western blot analysis of PIM1 (arrow) using PIM1 Antibody (C-term) (Cat# 102-17707). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIM1 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.