Anti-PIM2 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	11040
Protein Name / Synonyms	PIM2
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

PIM2 antibody, proto-oncogene Pim-2 (serine threonine kinase) antibody, serine/threonine-protein kinase pim-2 antibody, serine/threonine protein kinase pim-2 antibody, pim-2h antibody, pim-2 oncogene antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

34 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 53 and 278 of PIM2 (Uniprot ID# Q9P1W9)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Breast ca, using PIM2(107-11098) antibody at 1:250 dilution.

PIM2 antibody detects PIM2 protein by Western blot analysis.
A. 50 µg mouse testis lysate/extract
12 % SDS-PAGE
PIM2 antibody (107-11098) dilution: 1:500

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
107-11098 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

Featured Products