Anti-PIM2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9P1W9
Gene Id	11040
Gene Symbols	PIM2
Protein Name / Synonyms	PIM2 (C-term)
Clonality	Polyclonal
Preparation	This PIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-308 amino acids from the C-terminal region of human PIM2.
Concentration (lot specific)	1.640 mg/ml
Recommended Applications	Western Blotting, IHC-P, IP
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases. Pim-2 is highly homologous to Pim-1 with similar oncogenic functions. Pim-2 overexpression promotes resistance to a host of apoptotic stimuli; its expression is negatively regulated by growth factor depletion. Increased levels of Pim-2 has also been observed in certain cancers.

Specificity

Other Names

PIM2; Serine/threonine-protein kinase pim-2; Pim-2h

NCBI Accession #

NP_006866.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

34190

Recommended Dilutions

WB: 1:1000; IP: 1:50-100; IHC: 1:50-100

	Western blot analysis of anti-PIM2 Antibody (C-term) (Cat# 102-17709) in Hela cell line lysates (35ug/lane). PIM2 (arrow) was detected using the purified Pab.
	Western blot analysis of PIM2 (arrow) using rabbit polyclonal PIM2 Antibody (D292) (Cat# 102-17709). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIM2 gene.
	PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4;11 extract and used for the same in vitro phosphorylation reaction as a negative control.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products