Anti-PIST (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9HD26
Gene Id	57120
Gene Symbols	GOPC
Protein Name / Synonyms	PIST (Center)
Clonality	Polyclonal
Preparation	This PIST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the Central region of human PIST.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.

Specificity

Predicted Reactivity

Mouse

Other Names

GOPC; CAL; FIG; Golgi-associated PDZ and coiled-coil motif-containing protein; CFTR-associated ligand; Fused in glioblastoma; PDZ protein interacting specifically with TC10

NCBI Accession #

NP_001017408.1;NP_065132.1

Other Accession #

Q8BH60

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

50520

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of PIST Antibody (Center) (Cat# 102-17222) in A375 cell line lysates (35ug/lane). PIST (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PIST antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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