Anti-PITX2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q99697
Gene Id	5308
Gene Symbols	PITX2
Protein Name / Synonyms	PITX2 (C-term)
Clonality	Polyclonal
Preparation	This PITX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids from the C-terminal region of human PITX2.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Pilx2 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. It plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this protein are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development.

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish, Chicken, Xenopus

Other Names

PITX2; ARP1; RGS; RIEG; RIEG1; Pituitary homeobox 2; ALL1-responsive protein ARP1; Homeobox protein PITX2; Paired-like homeodomain transcription factor 2; RIEG bicoid-related homeobox transcription factor; Solurshin

NCBI Accession #

NP_000316.2;NP_001191326.1;NP_001191327.1;NP_001191328.1;NP_700475.1;NP_700476.1

Other Accession #

Q9I8K3; Q6QU75; Q9PWR3; Q9R0W1; P97474; Q9W5Z2; O93385

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

35370

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

Fluorescent image of Hela cell stained with PITX2 Antibody (C-term)(Cat# 102-12417/SA070209B).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PITX2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).PITX2 immunoreactivity is localized to Nucleus significantly.

Western blot analysis of anti-Pilx2 Pab (Cat# 102-12417) in CEM cell line lysates (35ug/lane). Pilx2(arrow) was detected using the purified Pab.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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