Anti-PKA 2 beta (PRKAR2B) (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P31323
Gene Id	5577
Gene Symbols	PRKAR2B
Protein Name / Synonyms	PKA 2 beta (PRKAR2B) (N-term)
Clonality	Polyclonal
Preparation	This PKA 2 beta (PRKAR2B) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-62 amino acids from the N-terminal region of human PKA 2 beta (PRKAR2B).
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.

Specificity

Other Names

PRKAR2B; cAMP-dependent protein kinase type II-beta regulatory subunit

NCBI Accession #

NP_002727.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

46302

Recommended Dilutions

WB: 1:1000

	Western blot analysis of anti-PRKAR2B Pab (Cat# 102-17101) in mouse brain tissue lysate. PRKAR2B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Western blot analysis of PRKAR2B (arrow) using rabbit polyclonal PRKAR2B Antibody (G46) (Cat# 102-17101). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAR2B gene.
	PRKAR2B Antibody (G46) (Cat# 102-17101) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PRKAR2B antibody detected the PRKAR2B protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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